限制酶Bsp63　I的化学修饰与抑制动力学研究

限制酶Bsp63　I的化学修饰与抑制动力学研究 CHEMICAL MODIFICATION AND INHIBITION KINETICS OF RESTRICTION ENDONUCLEASE BSp63I 董群 1 first-author 邹国林 1 曹新文 1 朱汝璠 1 武汉大学生命科学学院 武汉大学生命科学学院 COllege of Life Sciences, Wuhan University COllege of Life Sciences, Wuhan University 经亲和层析得到Ｂｓｐ６３Ｉ．用对氯汞苯甲酸、磷酸吡哆醛、２，３－丁二酮修饰该酶的巯基、赖氨酸、精氨酸残基，结果表明：这些基团均与该酶活性有关．动力学研究表明磷酸吡哆醛对酶的抑制是一种类反竟争性抑制． Restriction endonuclease Bsp63I has been isolated and purified by affinity chromatography. The role of specific amino acid residues in Bsp63I was determined by chemical modification. Sulfhydryl groups were modified with p-chloromercuribenzoic acid,lysineresidues with pyridoxal-5’-phosphate（PLP）and arginine residues with 2, 3-butanedione. Theresults show that these residues are related to activity of Bsp63I. Dynamic analysis showsthat the type of PLP inhibition is analogous anticompetitive inhibition. 限制性核酸内切酶 化学修饰 抑制类型 restriction endonuclease chemical modification inhibition type Q55 1996-02-01 2021-04-01 2