A rapid detection of hepatitis E virus（HEV） gene chip with nano-amplification technique was developed.The whole frame can be described with "sandwich hybridization of double probes coupled with silver stain enhancement".To detect Hepatitis E Virus

the virus RNA was extracted from the patient serum

then followed by RT-PCR.5′-end-NH2 modified oligonucleotides were designed to fabricate a DNA microarry in order to discriminate the target biotinylated DNA amplified by RT-PCR

the whole reaction system with partial dTTP was replaced by bio-dUTP

the signals generated with this method results from the precipitation of silver onto nanogold particles bound to streptavidin.The signals can be seen with naked eyes

which reduces the cost of detection.A total of 86 sera were tested by FQ-PCR and our homemade gene-chip.52 HEV positive samples tested by FQ-PCR all exhibit positively when tested by our rapid assay;These results suggested that chip-based detection method was consistent with a reference Fluorescence Quantitative PCR assay.