Ninety accessions of wild close relatives of barley

including 45 accessions from Tibet of China and 45 accessions from Middle East region

were studied on genetic diversity by using 27 SSR markers selected from seven barley linkage groups

of which eleven primer pairs of SSR markers have amplified polymorphic fragments effectively.A total of 126 allelic variants were produced

the average allelic variants per SSR primer pair being 11.45 with a range form 5 to 22.There are more than two alleles per locus in Tibet than that in Middle East region.Using the NTSYS and the POPGNEN 32 software

the similarity coefficients and the coefficient of genetic diversity were calculated respectively.And Dendrograms were generated by method of the unweighted pair group method arithmetic average（UPGMA）.The results show that China’s Tibetan wild close relatives of barley have higher genetic diversity than that in the Middle East region

and that offer a new evidence for the theory of the origin of barley in the East.