限制酶Sma　I参与的PCR产物克隆技术

限制酶Sma　I参与的PCR产物克隆技术 An EFFICIENT METHOD FOR CLONING OF PCR PRODUCTS BY Sma I-DIRECTED LIGATION 周荣家 1 first-author 郭一清 1 程汉华 1 余其兴 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 介绍了一种增加连接效率的ＰＣＲ产物克隆技术，即ＳｍａＩ内切酶参与的ＰＣＲ产物与ＳｍａＩ酶切的载体的连接；并通过克隆黄鳝基因组中一段约２５０ｂｐ的ＰＣＲ产物的实例证实了该技术的可靠有效性． This paper presents data demonstrating an efficient method to increase theyield of recombinants in cloning PCR products. In the example presented, 250 hp PCR product from amplification of MonoPterus albus genomic DNA was blunt-end ligated to a SmaI-cutPUC13 in presence of SmaI. 黄鳝 PCR扩增 亚克隆 Monopterus albus PCR amplification subcloning Q785 中国博士后科学基金,国家自然科学基金,武汉市青年科技晨光计划提供资助 1996-02-01 2021-04-01 2