吖啶橙-细胞DNA荧光抑制法初步筛选抗癌药物的研究

吖啶橙-细胞DNA荧光抑制法初步筛选抗癌药物的研究 PRELIMINARY SCREENING OF ANTICANCER DRUGS BY ACRIDINE ORANGE CELLULAR DNA FLUORESECENCE INHIBITION 唐宏武 Tang Hongwu 1 2 3 first-author 陈蓓 Chen Bei 1 2 3 陈观铨 Chen Guanquan 1 2 3 叶勇 Ye Yong 1 2 3 曾云鹗 Zeng Yune 1 2 3 沈昊宇 1 2 3 武汉大学化学系分析测试科学系 武汉大学化学系分析测试科学系 Department of Chemistry, Department of Analysis and Measurement Science, Wuhan University, Wuhan 430072,China) Shen Haoyu Department of Chemistry, Department of Analysis and Measurement Science, Wuhan University, Wuhan 430072,China) Shen Haoyu 华中师范大学化学系 华中师范大学化学系 武汉大学化学系分析测试科学系华中师范大学化学系 武汉大学化学系分析测试科学系华中师范大学化学系 以二倍体鼠肝细胞为标准细胞，用荧光探针吖啶橙（ＡＯ）示踪药物／细胞ＤＮＡ的作用而提出了一种用Ｈａｄａｍａｒｄ变换显微荧光图象分析法初步筛选抗癌药物的新方法．对Ｄ－氨基葡萄糖的３ｄ过渡金属的配合物、Ｄ－氨基葡萄糖与β－萘酚醛的Ｓｃｈｉｆ碱及其与３ｄ过渡金属的配合物和它们分别与甘氨酸形成的混配物共四个系列２２种化合物与细胞ＤＮＡ的作用进行了研究，结果表明：Ｃｏ（Ⅲ）ＮＧ，Ｃｏ（Ⅱ）ＮＧ，ＣｕＮＧ，ＣｕＧｌｕ，ＮｉＮＧ，ＣｕＧｌｕＧ，Ｆｅ（Ⅲ）ＮＧ７种化合物有明显的抗癌活性，其中ＣｕＧｌｕ对Ｇ０期细胞更为敏感． Using rat liver 2c hepatocyte as standard cell and acridine orange （AO） to trace the interaction of drug and cellular DNA, a novel method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was developed. The study of Schiff base ligands of 2 hydroxy naphthaldehyde with D glucosamine （NG） and the complexes of 3 d transition metal ions with D glucosamine （Glu） and mixed ligand complexes of NG and Glu series complexes with α glycine was performed, the results shows that: Co（Ⅲ）NG 、Co（Ⅱ）NG、CuNG、CuGlu、NiNG、CuGluG and Fe（Ⅲ）NG have obvious anticancer activity, among them, CuGlu is the most sensitive for G 0 phase cell. Hadamard变换显微荧光图象分析 鼠肝细胞 AO／细胞DNA体系 Schiff碱配合物 抗癌活性 Hadamard transform microscopic image analysis rat liver hepatocyte AO/cellular DNA system Schiff base complex anticancer activity O657.3 国家自然科学基金,湖北省自然科学基金 1997-06-01 2021-04-01 6