黑卵蜂寄主识别利它素的性质研究

黑卵蜂寄主识别利它素的性质研究 ISOLATION AND CHARACTERIZZATION OF THE HOST RECOGNITION KAIROMONE OF TELENOMUS SPP. 邓莉 Deng Li 1 first-author 罗志良 Luo Zhiliang 1 戴开甲 Dai Kaijia 1 曹爱华 Cao Aihua 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences,Wuhan University College of Life Sciences,Wuhan University 通过盐析、柱层析和分子筛层析，从松毛虫雌蛾附腺中分离到一种ＳＰ－Ｉ组份，经生化方法检测鉴定，此组份是一种糖蛋白，分子量为１．３５×１０５，等电点为４．２，蛋白质含量９２％，糖含量８％．它能引诱松毛虫黑卵蜂和松茸毒蛾黑卵蜂在非天然寄主上的产卵检查、刺探行为，是一种黑卵蜂寄主识别利它素． A compound named SP Ⅰwas isolated from the extract of the female Dendrolimus punctants and partially putified by （NH 4） 2SO 4 Salting out,CM C cation exchange column chromatography,Sephadex G 200 gel filtration.It was obtained clearly in one band in SDS PAGE.The molecular weight of the compound was 1.35×10 5;it was a acidic glucoprotein（pI=4.2）,and contained 92% protein,8% carbohydrate. The bioassay results revealed that it could induced Telenomus dendrolimusi and T.dasychiri Chen et Wu to examine and to probe the artiticial host egg coated with SP Ⅰ,the host recognition kairomone. 松毛虫 附腺 黑卵蜂 寄主识别利它素 糖蛋白 dendrolimus punctatus accessory gland Telenomus sp. host recognition kairomone glucoprotein S476.3 国家自然科学基金(61672102) 1997-06-01 2021-04-01 6