A cell line was established from pupal ovary of Philosamia cynthia ricini （Pcr） and was designatet as WVB Pcr 02. The cell line WVB Pcr 02 was established from pupal ovarian tissues of Philosamia Cynthia ricini . The cell line has been transfered for 102 passages in 36 months. The medium BML TC100 supplemented with 20% Fetal calf serum was suitable for culturing pupal ovaries of Philosamia Cynthia ricini and Tc Tryptose broth has the ability to stimulate the growth of the silkworm cells.It showed the same character as other cell of silkworm in the process of growth. suspension containing cell masses and single cells was subcultured with the split ratio of 1∶2.The cells population doubling time was 60～64 h and the maximum cell population per milliliter was 4.3 times as the initiated cell density. The homologous PcrNpv was used to infect cell line. Polyhedral inclusion bodies formed in the nuclei of the WVB Pcr 02 cell line

which was susceptible to the virus. Ovary 90% of cells were infected. Product dosages of 2.4×10 7 PIB/mL. When viewed with an electron microscope

the infected cells at five days post inoculation showed viral matrix in their nuclei. Nucleocapsids were formed in the matrix and were enveloped multiply as bundles of virus rods. This cell line was found to susceptible Pcr NPV. [WT5HZ〗