伪狂犬病毒表达载体的构建

伪狂犬病毒表达载体的构建 CONSTRUCTION OF PRV EXPRESSING VECTOR AND IDENTIFICATION WITH E.coli β LacZ 丁建华 Ding Jianhua 1 first-author 付烈振 Fu Liezhen 1 王家富 Wang Jiafu 1 罗满林 Luo Manling 1 谢小虎 Xie Xiaohu 1 张楚瑜 Zhang Chuyu 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 利用伪狂犬病毒（Ｐｓｅｕｄｏｒａｂｉｅｓｖｉｒｕｓ，ＰＲＶ）湖北地方株胸腺核苷激酶（ＴＫ）基因和ｇＧ糖蛋白基因启动子（ＰｇＧ），构建了ＰＲＶ基因转移载体．通过β－半乳糖苷酶基因确定ＰｇＧ控制下外源基因的表达并筛选出表达β－半乳糖苷酶的ＰＲＶ重组病毒．采用ＰＣＲ对重组病毒进行了初步鉴定，证实外源基因可由构建的转移载体导入病毒ｔｋ基因中，重组的ＰＲＶ湖北地方株可用于表达外源基因． The expression of β lacZ controlled by PRV glycoprotein gG promoter（PgG）, with partial tk sequences of PRV strain HS9304 on both sides, was confirmed. And PRV recombinant expressing β lacZ was produced by insertion of PgG β lacZ into tk gene of PRV strain HS9304. The results indicate that PRV strain HS9304 has the potential to be used as gene expressing vector. A transfer vector was constructed successfully with PgG and tk sequences which can be used to transfer foreign gene into viral genome. 伪狂犬病毒 gG糖蛋白基因启动子（PgG） 胸腺核苷激酶基因 PRV glycoprotein gG promoter（PgG） thymidine kinase gene（tk） S852.65 国家自然科学基金,湖北省科委基金,武汉市科委基金 1997-06-01 2021-04-01 6