Plasmid pBI211-cat was transferred into D.salina via electroporation and was proved transiently expressed.Vector pBIRNAI-Dsa was constructed from pBI211-cat and transformed into Dunalilla salina inducing pds gene silencing.Two modes

high-voltage and low-pitched of 3-7 kV/cm and low-voltage and high-tension of 300-500 V/cm

were adopted to transform cells and the results were detected from different aspects.PCR amplification and GUS histochemistry staining also proved that pBI221cat had been delivered into cells and expressed transiently.Partial pds sense sequence and its reverse were inserted into pBI221-cat to form the RNAi vector pBIRNAI-Dsa.Pds gene expressing of the treated group transformed with pBIRNAI-Dsa was reduced

even to 28% of the control group

suggesting that pds gene expressing was restrained.