kinds of restriction endonucleases have been used to analyze the mtDNA from liver of Ctenopharyngodon idellus .The molecular weight of C.idellus mtDNA is 10.25×10 6 daltons about 16.58 kilobase pairs.The recognition sites of PstⅠ
BamHⅠ
XbaⅠ
BglⅠ
HindⅢ
BglⅡ
PvuⅡ
XhoⅠ
EcoRⅠ and SalⅠ on this mtDNA are 2
3
3
4
7
3
6
2
4 and 0 respectively.The restriction maps of C.idellus mtDNA have been established based on the number and size of those fragments obtained by single and double digestions.