水溶液中β-环糊精与苯甲酸雌二醇包络反应的光谱研究与应用

水溶液中β-环糊精与苯甲酸雌二醇包络反应的光谱研究与应用 SPECTROSCOPIC STUDY AND APPLICATION ON THE INCLUSION COMPLEXATION OF β CYCLODEXTRIN WITH ESTRADIOL BENZOATE IN AQUEOUS SOLUTION 姚礼峰 Yao Lifeng 1 first-author 罗兆福 Luo Zhaofu 1 马红燕 Ma Hongyan 1 潘祖亭 Pan Zuting 1 武汉大学化学系 武汉大学化学系 Department of Chemistry, Wuhan University Department of Chemistry, Wuhan University 在硫酸酸性介质和溴化十六烷基三甲铵（ＣＴＭＡＢ）存在下用紫外吸收、荧光光谱法对β－环糊精（β－ＣＤ）与苯甲酸雌二醇（ＥＢ）的主客体包络反应及协同增敏作用机理进行了研究，提出了水溶液中测定ＥＢ的荧光光度新方法，灵敏度高，检出限为４．９×１０－９ｍｏｌ·Ｌ－１，其线性范围为５．３×１０－９～２．０×１０－６ｍｏｌ·Ｌ－１，方法的相对标准偏差为１．３％（ｎ＝５）．应用本法于苯甲酸雌二醇注射液样品中ＥＢ的测定，结果满意 The inclusion complexation reaction of β cyclodextrin with estradiol benzoate （EB） in the presence of cetyltrimethylammonium bromide has been studied by means of UV absorption and fluorescent spectrometry. The reaction conditions, the formation constant, the mechanism of the host guest inclusion complex have been studied and a simple, highly sensitive fluorescent synergistic method for the determination of EB has been established with satisfactory results. β－环糊精 苯甲酸雌二醇 溴化十六烷基三甲铵 光谱分析 包络物 β cyclodextrin estradiol benzoate cetyltrimethylammoinum bromide spectral analysis inclusion complexation O657.39 国家自然科学重点基金 1997-06-01 2021-04-01 6