基因免疫诱导小鼠对狂犬病病毒糖蛋白的免疫反应

基因免疫诱导小鼠对狂犬病病毒糖蛋白的免疫反应 A BIBLIOMETRICS ANALYSIS OF JOURNAL OF WUHAN UNIVERSITY（NATIONAL SCIENCE EDITION） 1990￣1995 怀济森 Zhan Yuqing 1 2 3 first-author 陈焕春 He Shaozhen 1 2 3 熊远著 Wang Caixia 1 2 3 武汉大学生命科学学院 武汉大学生命科学学院 Library,Wuhan University,Wuhan 430072, Chian Library,Wuhan University,Wuhan 430072, Chian 华中农业大学畜牧医学院 华中农业大学畜牧医学院 武汉大学生命科学学院华中农业大学畜牧医学院 武汉大学生命科学学院华中农业大学畜牧医学院 将狂犬病病毒糖蛋白基因（Ｒｇｐ）按正确方向插入真核表达载体ｐＫＳＶ１０及ｐＭＴ０１０／Ａ＋中相应启动子下游，构建成由ＳＶ４０早期启动子启动的ｐＫＳＶＲｇｐ及羊金属硫蛋白启动子启动的ｐＭＴＲｇｐ两种狂犬病病毒糖蛋白表达载体．将两种重组体分别经小鼠两侧腿部肌肉按不同方案直接注射到１２只６～８周龄小鼠体内，成功地在注射小鼠血清内检测到抗狂犬病病毒抗体，而且注射２次的小鼠ＥＬＩＳＡ抗体滴度显著提高，并且ｐＫＳＶＲｇｐ诱生的抗体滴度更高． Taking the 741 articles published in the Journal of Wuhan University （ National Science Edition） 1990￣1995 as the information sources, a statistical analysis is made on papers. authors. collaboration and citation in this journal. The results show that the percentage of funded paper was 52.50%, the arerage time lag for published paper was 13.59 monthes ,the average number of anther for per article was 3.097 , the percentage of collaborating article was 84.1%,the average number of citations for per article was 7.57, the percentage of the articles without citations was 0.4%, the percentage of the article with author’s self citations was 53.04%,the percentgae of the journal’s self citations was 5.03%,61.95% of citations were from foreign language ,77.33% of citations were from periodicals 狂犬病病毒 糖蛋白基因 注射 抗体 S852.655 国家教委博士点基金 1997-04-01 2021-04-01 4