Western blot showed that human cervical cancer cell lines CaSki

Hela S3 and SiHa were capable of expressing certain amount of HPV E7 proteins when anti-HPV E7 mAb was used.The amount of E7 protein in CaSki was higher as compared with that in Hela S3 or SiHa.SiHa showed much less amount of E7 protein among three cell lines.Hence

CaSki was chosen for further investigation in order to understand whether the anti-HPV E7 antibody interacted with those cervical cancer cells containing HPV DNA.The investigation by immunofluorescence microscopy revealed that the anti-HPV E7 mAb was unable to interact with those cells that were thriving

but targeted those cells that were dying or damaged by Triton X-100 treatment and 137Cs irradiation.Flow cytometry gave a net-increased value of 16.5% to the interaction between the anti-HPV E7 mAb and CaSki cells as compared with that for the isotype HBsAg antibody.High percentage of cells binding to the FITC-conjugated antibody indicated the specific interaction between the anti-HPV E7 mAb and the E7 protein inside CaSki cells.