Myristylated membrane protein（MMP） gene was cloned and identified from Rana grylio virus（RGV）.It is 972 bp long and encodes a protein of 323 aa with a predicted molecular mass of 35×103.Alignment of the deduced amino acid sequence of RGV MMP showed that they had the homology degree of more than 64% with other Ranavirus.The recombinant prokaryotic expression plasmid containing this fragment was constructed and expressed an expected 53×103 fusion protein.The antiserum of MMP protein was produced.Its temporal expression pattern during RGV infection course was characterized by RT-PCR and Western blot analysis.It begins to transcribe as early as 4 h postinfection（p.i.） and translate after 6 h p.i.

which remains detectable at 48 h p.i.Using drug inhibition analysis by a de novo protein synthesis inhibitor（cycloheximide） and a viral DNA replication inhibitor（cytosine arabinofuranoside）

RGV mmp was classified as a late（L） viral gene during the in vitro infection.