pH敏感相分离的固定化酶的制备和性质

pH敏感相分离的固定化酶的制备和性质 STUDIES ON THE MELT COPOLYMERIZATION OF PHOSPHORUS CONTAINING DIACID AND BIS（p CARBOXYPHENOXY） PROPAND FOR DDS 贺枫 Fu Jie 1 first-author 卓仁禧 Zhuo Renxi 1 刘立建 Fan Changlie 1 徐勉懿 1 武汉大学化学系国家教委生物医用高分子材料开放实验室 武汉大学化学系国家教委生物医用高分子材料开放实验室 Department of Chemistry,Wuhan University,Wuhan 430072,China) \ \ Department of Chemistry,Wuhan University,Wuhan 430072,China) \ \ 报道了一种新的制备溶解性可调节的固定化酶的方法．先将木瓜蛋白酶与Ｎ－羟基琥珀酰亚胺丙烯酸酯（ＮＡＳ）反应，再利用自由基溶液聚合反应，制备了ｐＨ敏感相分离的固定化木瓜蛋白酶．考察了固定化过程中的ｐＨ值、反应时间及给酶量对固定化木瓜蛋白酶活力的影响，同时还测定了单体配比对固定化酶临界ｐＨ值的影响．这些固定化酶均具有较敏感相分离的特性，兼有溶液酶和固相酶两者的优点． Phosphorus containing copolyanhydrides DECP CPP and DCPP CPP were synthesized by melt copolymerization of bis（p carboxy phenyloxy ethyl）, ethyl phosphate （DCPE）, bis（p carboxyphenyloxy ethyl）, phenyl phosphate （DCPP） and bis（p carboxyphenoxy）propane acid（CPP）. The copolyanhydrides were characterized by 1H NMR, IR spectrometry. In Vitro degradation of copolyanhydrides in phosphate buffer solution at 37℃ were determined. Drug release profile of Rifampicinum （RFP） were also studied. \ \ pH敏感 可逆溶解－沉淀性 相分离 固定化酶 copolyanhydride polyphosphate drug controlled release RFP O629.8 国家教委博士点基金 1997-04-01 2021-04-01 4