水稻花药特异表达基因启动子的扩增及克隆

水稻花药特异表达基因启动子的扩增及克隆 EFFECT OF DESICCATION ON DIFFERENTIATION FREQUENCY OF GREEN PLANTLETS AND BIOCHEMICAL PROPERTIES OF CALLI OF WILD RICE 张晓国 Tan Guangxuan 1 2 3 first-author 刘玉乐 Shu Lihui 1 2 3 康良仪 Yuan Wenjin 1 2 3 朱英国 Liao Lanjie 1 2 3 田波 He Guangcun 1 2 3 中国科学院微生物研究所 中国科学院微生物研究所 College of Life Sciences,Wuhan University College of Life Sciences,Wuhan University 武汉大学生命科学学院 武汉大学生命科学学院 中国科学院微生物研究所武汉大学生命科学学院 中国科学院微生物研究所武汉大学生命科学学院 采用ＤＮＡ聚合酶链式反应（ＰＣＲ）扩增技术，以水稻黄化苗总ＤＮＡ为模板成功地扩增到水稻花药特异表达基因启动子Ｏｓｇ６Ｂ的７７０ｂｐ和９６０ｂｐ两个片段Ｏｓｇ６Ｂａ和Ｏｓｇ６Ｂｂ．并将其克隆到ｐＵＣ１８上形成完整的Ｏｓｇ６Ｂ，这为通过基因工程方法进行水稻杂种优势利用奠定了基础． Calli of four wild rices were desiccated in a petri dish with a single layer of filter paper prior to transfer to the regeneration medium.The plant regeneration frequencies of three wild rices were increased at different degrees.Desiccation of calli resulted in the signifcant declining of indole 3 acetic acid（IAA） and water soluble protein （WSP）content,but activity of peroxidase iszyme was increased. With desiccation,the increasing of activity of peroxidase iszyme might result in the decreasing of IAA content,and improved plant regeneration frequency of calli of wild rice. 启动子 PCR扩增 水稻 Q503 国家攀登计划,国家863高技术项目 1997-04-01 2021-04-01 4