N-羟基琥珀酰亚胺-3-吲哚乙酸酯柱前衍生HPLC分离荧光检测氨基酸

N-羟基琥珀酰亚胺-3-吲哚乙酸酯柱前衍生HPLC分离荧光检测氨基酸 DETERMINATION OF AMINO ACIDS AS THEIR N HYDROXY SUCCINIMIDYL 3 INDOLYLACETATE DERIVATIVES BY PRE COLUMN DERIVATIZATION HPLC SEPARATION AND FLUORESCENCE DETECTION 金虹 Jin Hong 1 first-author 刘勋 Liu Xun 1 王红 Wang Hong 1 张华山 Zhang Huashan 1 程介克 Cheng Jieke 1 武汉大学化学学院 武汉大学化学学院 College of Chemistry, Wuhan University College of Chemistry, Wuhan University Ｎ－羟基琥珀酰亚胺－３－吲哚乙酸酯（ＳＩＩＡ）是一种含有新型反应活性基团的荧光衍生试剂，可在温和的条件下与氨基化合物进行衍生反应．以ＳＩＡ为柱前衍生试剂，研究了其氨基酸衍生物的高效液相色谱行为和分离条件，在含适宜ｐＨ值柠檬酸（Ｈ３ｃｉｔ）－Ｎａ２ＨＰＯ４缓冲溶液的甲醇－水流动相中，获得了常见氨基酸的有效分离，检出限达亚ｐｍｏｌ级． N hydroxy succinimidyl 3 indolylacetate （SIIA） is a new fluorescence derivatization reagent with an indole ring and a N hydroxy succinimide ester reactive function. It can react with amino compounds under mild conditions and yields corresponding amides, which have strong fluorescence at λ ex / λ em =278 nm/355 nm. This paper covers the reversed phase HPLC separation and determination of sixteen derivatized amino acids with SIIA by a mobile phase of methanol water containing H 3cit Na 2HPO 4 buffer. The excess reagent will be rapidly hydrolyzed to give 3 indolylacetic acid which can be easily separated from derivatives, so it does not interfere with the analysis. The study showed that SIIA can be used as a derivatization reagent for the analysis of amino compounds by reversed phase HPLC and fluorescence detection. N－羟基琥珀酰亚胺－3－吲哚乙酸酯 荧光衍生试剂 氨基酸 高效液相色谱 N hydroxy succinimidyl 3 indolylacetate fluorescence derivatization reagent aminoacid high performance liquid chromatography （HPLC） O629.71;O657.32 国家自然科学基金(61672102) 1998-02-01 2021-04-01 2