粘虫核型多角体病毒pdv-e66基因的序列测定及比较研究

粘虫核型多角体病毒pdv-e66基因的序列测定及比较研究 THE SEQUENCING OF pdv e66 GENE FROM LEUCANIA SEPARATA NUCLEAR POLYHEDROSIS VIRUS AND ITS COMPARISON WITH OTHER BACULOVIRUSES 金天权 Jing Tianquan 1 first-author 齐义鹏 Qi Yipeng 1 金海陵 Jing Hailing 1 黄永秀 Huang Yongxou 1 苏霏 Su Fei 1 武汉大学病毒研究所 武汉大学病毒研究所 Institute of Virology,Wuhan University Institute of Virology,Wuhan University 测定了ＬｓＮＰＶｐｄｖ－ｅ６６基因１８９６ｂｐ序列，与其它杆状病毒ｐｄｖ－ｅ６６基因进行了同源性比较，其与ＡｃＮＰＶｐｄｖ－ｅ６６基因核苷酸同源性为５１．９％，氨基酸同源性为３８．８％，分析指出，ＬｓＮＰＶｐｄｖ－ｅ６６基因中有５个高度保守区和一个ＮＴＳ．５端具有典型的晚期基因启动子特征． A 3 443 bp fragment from LsNPV genome,including pdv e66 gene and another ORF, was sequenced. The pdv e66 genes from four kinds of baculoviruses were compared. Two conserved late transcriptional motifs TAAG were found in LsNPV pdv e66 gene,similar to those in other pdv e66 genes. The LsNPV pdv e66 ORF is 204 base pairs shorter than the AcNPV pdv e66 ORF at the 5 end. The other ORF can code a 12×10 3 protein, which has the homology with AcNPV ORF108. LsNPVpdv－e66基因 序列测定 AcNPV LsNPV pdv e66 sequencing AcNPV Q78 国家自然科学基金(61672102) 1998-02-01 2021-04-01 2