酵母细胞壁主要结构蛋白CWP33的纯化与研究

酵母细胞壁主要结构蛋白CWP33的纯化与研究 PURIFICATION AND CHARACTERIZATION OF MAJOR STRUCTURAL PROTEIN OF CELL WALL（CWP33）OF YEAST 张西平 Zhang Xiping 1 first-author 黄昆 Huang Kun 1 黄磊 Huang Lei 1 张楚瑜 Zhang Chuyu 1 王鄂生 Wang Esheng 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 在酵母Ｃａｎｄｉｄａｕｔｉｌｉｓ细胞壁上发现一种细胞壁主要结构蛋白（ＣＷＰ３３），其分子量为３３×１０３，与其它细胞壁蛋白不同，该蛋白在细胞壁上对胰蛋白酶作用不敏感，而其它细胞壁蛋白组分几乎完全被胰蛋白酶降解．利用这一性质将该蛋白从细胞壁上抽提后，经ＤＥＡＥ－纤维素离子交换柱层析和Ｓｅｐｈａｒｏｓｅ凝胶层析得到纯化．经研究ＣＷＰ３３蛋白具有葡聚糖内切酶活性，可以水解地衣糖，但不能水解对硝基苯葡萄糖苷．该蛋白可能具有重要的结构功能． A major structural protein（CWP33） with molecular mass of 33×10 3 was discovered in cell wall of Candida utilis .It was quite different from all the other cell wall proteins,it was not sensitive to action of trypsin, while other cell wall proteins were hydrolyzed almost completely. According to this quality,the protein was extracted from cell wall and was purified by DEAE cellulose chromatography and Sepharose 4B gel filtration.This protein was found to have endo glucanase activity,it could hydrolyze lichenan, but not p nitrophenyl glucose.This protein may play some important roles in cell wall structure. Candidautilis 细胞壁 主要结构蛋白 Candida utilis cell wall major structural proteins Q51 国家自然科学基金(61672102) 1998-02-01 2021-04-01 2