溶解-沉淀可逆的酶载体的合成及其应用

溶解-沉淀可逆的酶载体的合成及其应用 SYNTHESIS AND APPLICATION OF pH SENSITIVE IMMOBILIZED ENZYME CARRIERS WITH REVERSIBLE SOLUBILITY PRECIPITATION PROPERTY 柏正武 Bai Zhengwu 1 first-author 卓仁禧 Zhuo Renxi 1 武汉大学化学学院教育部生物医用高分子材料开放实验室 武汉大学化学学院教育部生物医用高分子材料开放实验室 College of Chemistry, Wuhan University College of Chemistry, Wuhan University 由丙烯酸、甲基丙烯酸甲酯和顺丁烯二酸酐共聚，合成了ｐＨ敏感的溶解－沉淀可逆的共聚物，并测试了共聚物溶解－沉淀相分离的临界ｐＨ值．以这种共聚物为载体，进行了木瓜蛋白酶的固定化． pH sensitive terpolymers with reversible solubility precipitation property when the pH values of the solution were changed were synthesized by terpolymerization of acrylic acid, methyl methacrylate and maleic anhydride. The chemical structure of these terpolymers obtained was characterized by IR spectrum. The critical pH values of solubility precipitation of these terpolymers were investigated. Immobilization of papain on these polymers were also carried out. pH敏感高分子 可逆溶解－沉淀 固定化酶 pH sensitive polymer reversible solubility precipitation immobilized enzyme Q55 国家自然科学基金(61672102) 1998-04-01 2021-04-01 4