受乳糖启动子控制的基因表达过程的优化

受乳糖启动子控制的基因表达过程的优化 OPTIMIZATION OF THE GENE EXPRESSION BY INDUCING LAC PROMOTOR REGULATED GENE WITH LACTOSE 侯松旺 Hou Songwang 1 first-author 李小洁 Li Xiaojie 1 马辉文 Ma Huiwen 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 借助于ＳＤＳ－聚丙烯酰胺凝胶电泳分析方法，对乳糖诱导的由乳糖启动子控制的大肠杆菌ＲＮＡ聚合酶σ７０因子基因表达过程进行了优化研究．结果表明，含此融合基因的大肠杆菌菌株ＢＬ２１（ＤＥ３）ｐＧＥＭＤ的生长量达到ＯＤ６００ｎｍ＝１．０时，用终浓度为０．０８％的乳糖进行连续５ｈ的诱导，可使大肠杆菌ＲＮＡ聚合酶σ７０因子基因的表达量达到最大，并达到ＩＰＴＧ同样的诱导效果．流加低浓度的乳糖溶液能增加σ７０因子的合成量，但是促进作用不很显著． The optimization of lactose induced over expression of the cloned Escherichia coli RNA polymerase σ 70 factor gene in Escherichia coli strain BL21（DE3） was reported. It was found that the expression level of σ 70 factor gene inserted on a phagemid pGEMEX, was the highest when the strain BL21（DE3） harboring the phagemid was induced for 5 h with 0.08% of lactose at a growth level of OD 600 nm = 1.0. Feeding lactose solution in a lower concentration at an interval of 30 min. into the bacterial culture at the optimal growth level could slightly promote the synthesis of σ 70 factor. 乳糖诱导作用 乳糖启动子 基因表达 lac promotor gene expression lactose as an inducer Q78 国家教委留学回国人员基金 1998-04-01 2021-04-01 4