中国人CD59cDNA克隆、序列分析及其表达

中国人CD59cDNA克隆、序列分析及其表达 cDNA SYNTHESIS,CLONING, SEQUENCING AND EXPRESSION OF CD59 黄健 Huang Jian 1 first-author 苟德明 Gou Deming 1 郑从义 Zhen congyi 1 夏总平 Xia Zongping 1 李文鑫 Li Wenxin 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences,Wuhan University College of Life Sciences,Wuhan University 用ＲＴ－ＰＣＲ方法从中国人胚胎总ｍＲＮＡ中扩增出４２０ｂｐ人补体终末阶段同源限制因子（ＣＤ５９）的ｃＤＮＡ，序列分析结果表明，所获得的ＣＤ５９ｃＤＮＡ与文献报道中的基因序列完全一致，含ＣＤ５９全长编码区．构建了真核表达质粒，在３Ｔ３细胞中得到了表达． CD59 is a 20×10 3 homologogous restriction factor which inhibits the assembly of homologous complement membrane attack complexes.A human CD59 cDNA containing full encoding region was obtained by RT PCR from chinese human embryo.The target gene was cloned and sequenced. The data indicated that it was identical to that of natural one. After transformed into 3T3 cell, the protein of CD59 expressed by the cell has been observed. 同源限制因子 RT－PCR 序列分析 真核细胞表达 CD59 RT PCR DNA sequencing eukaryotic cell expression Q786 湖北省科委“九五”攻关资助 1998-06-01 2021-04-01 6