σ⁷⁰因子抗体能特异性地识别其他转录因子

σ 70 因子抗体能特异性地识别其他转录因子 ANTIBODIES AGAINST σ 70 FACTOR SPECIFICALLY RECOGNIZE THE σ FACTORS FROM OTHER MICROORGANISMS 侯松旺 Hou Songwang 1 first-author 龚睿 Gong Rui 1 马辉文 Ma Huiwen 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 通过免疫印迹法研究了抗大肠杆菌ＲＮＡ聚合酶σ７０因子的抗体对产气杆菌、金黄色葡萄球菌和不同生长阶段的天蓝色链霉菌细胞内蛋白质的免疫识别作用．结果表明，抗大肠杆菌ＲＮＡ聚合酶σ７０因子抗体能特异性地识别上述微生物细胞内的一些蛋白质．对这些蛋白质的属性作了讨论，认为它们是ＲＮＡ聚合酶的转录因子．本研究还发现，抗大肠杆菌ＲＮＡ聚合酶σ７０因子抗体识别的产气杆菌细胞内的蛋白质，在数量和种类上几乎完全与大肠杆菌相同． The immunoreactivity of the antibodies directed against σ 70 factor of Escherichia coli RNA polymerase to the cellular protein from the cell lysate of E.coli, Bacterium aerogenes, Staphylococcus aureus , and Streptomyces coelicolor was studied by means of Western bolt. It was found that the antibodies could specifically recognize some cellular proteins of the aforementioned microoganisms. The essentials of the proteins recognized by anti sigma 70 factor sera was discussed. They were considered as transcirption factors of the RNA polymerase. It was also found that the proteins from Bacterium aerogenes immunoreacted with the antibodies to the sigma 70 factor were similar as that of the proteins from E.coli , either in the immuno reactivity and in the pattem of molecular weight. 抗σ70因子抗体 免疫识别 转录因子 antibodies to sigma 70 factor transcription factor immuno recognization R392.12 国家教委留学回国人员科研基金 1998-06-01 2021-04-01 6