隐藏嗜酸菌DX1-1产PHB条件的优化

隐藏嗜酸菌DX1-1产PHB条件的优化 Optimization of Culture Condition for PHB Accumulating by Acidiphilium cryptum DX1-1 徐爱玲 XU Ailing 1 first-author 历丽 LI Li 1 张帅 ZHANG Shuai 1 杨宇 YANG Yu 1 夏金兰 XIA Jinlan 1 中南大学资源加工与生物工程学院/生物冶金教育部重点实验室 中南大学资源加工与生物工程学院/生物冶金教育部重点实验室 School of Minerals Processing and Bioengineering,Central South University/Key Laboratory of Biometallurgy of Ministry of Education School of Minerals Processing and Bioengineering,Central South University/Key Laboratory of Biometallurgy of Ministry of Education 本试验对可积累聚β-羟基丁酸酯（PHB）的隐藏嗜酸菌（Acidiphilium cryptum）DX1-1菌株的培养条件进行了优化.确定了最佳碳氮源组合（葡萄糖,KNO3）,正交试验确定了最优培养条件即碳源浓度为40 g/L、氮源浓度为15 g/L以及初始pH值为3.0,并确定了在最优培养条件下生长曲线和PHB产量随时间的变化曲线.通过核磁共振对该菌胞内PHB的结构进行了分析.此次试验提高了DX1-1菌株PHB的产量,最高达到19.75 g/L. This experiment optimized the culture condition of Acidiphilium cryptum DX1-1,which was capable of producing poly-β-hydroxybutyrate（PHB）.Glucose and KNO3 acted as the best carbon and nitrogen substrates for the production of PHB.The L16（43） orthogonal experiment improved the yield when 40 g/L glucose as carbon source,15 g/L KNO3 as nitrogen source and pH 3.0 were adopted.Then the cells growth curve and PHB production curve were measured under the optimum culture condition.It is found that the process of PHB accumulating was a growth-associated process.NMR analysis further supported that the product accumulated by that strain had the typical structure of PHB.The PHB production of strain DX1-1 increased to 19.75 g/L. 隐藏嗜酸菌 生物塑料 聚-β-羟基丁酸酯 培养条件 Acidiphilium cryptum DX1-1 bioplastic poly-β-hydroxybutyrate incubating condition Q93 国家重点基础研究发展计划（973）项目（2004CB619201）;国家创新研究群体自然科学基金资助项目（50321402） 2008-06-01 2021-04-01 6 54