免疫球蛋白阳性细胞在大菱鲆免疫相关组织中的分布

免疫球蛋白阳性细胞在大菱鲆免疫相关组织中的分布 Distribution of Immunoglobulin-Positive Cells in Immune-Associated Tissues in Turbot（Scophthalmus maximus L.） 冯守明 FENG Shouming 1 first-author 绳秀珍 SHENG Xiuzhen 1 战文斌 ZHAN Wenbin 1 中国海洋大学水产学院/海水养殖教育部重点实验室 中国海洋大学水产学院/海水养殖教育部重点实验室 College of Fisheries,Ocean University of China/Key Laboratory of Mariculture Ministry of Education College of Fisheries,Ocean University of China/Key Laboratory of Mariculture Ministry of Education 采用单克隆抗体免疫组化标记、间接荧光标记法（IFAT）和流式细胞术（FACS）,对大菱鲆免疫相关组织中免疫球蛋白（Ig）阳性细胞进行了检测.免疫组化标记后发现,鳃组织中Ig阳性细胞存在于鳃小片上皮细胞与支持细胞之间的间隙中,后肠Ig阳性细胞存在于固有层和粘膜下层中,外周血血涂片中可见Ig阳性细胞,头肾Ig阳性细胞则无规律地散布于肾组织中.间接荧光标记和流式细胞仪分析免疫相关组织中分离的白细胞,发现大菱鲆不同免疫相关组织中Ig阳性细胞的数量不同,Ig阳性细胞数量分别为:鳃（1.60±0.71）%,后肠（3.91±1.06）%,外周血（28.95±0.39）%,头肾（21.54±0.90）%.结果表明,大菱鲆的免疫相关组织（含粘膜组织）具备一定的完成特异性免疫的细胞基础,且不同组织之间的免疫细胞数量存在显著差异. The methods of immunohistochemistry,indirect fluorescent antibodg Test（IFAT）and fluorescence-activated cell sorter（FACS） were used to determine and measure immunoglobulin（Ig） positive cells in immune-associated tissues of turbots.Results were as follows: Ig-positive cells were located in filament sinusoid between the epithelial cells and the supporting cells of gill,and were located in the tunica propria and the submucosa of hindgut.The Ig-positive cells also appeared in the smear of peripheral blood（PBL） and scattered in interstitial tissue of head kidney.The number of Ig-positive cells was different among tissues in turbot.The percentages of Ig-positive cells respectively were（1.60±0.71）% in gills,（3.91±1.06）% in hindgut,（28.95±0.39）% in PBL,（21.54±0.90）% in kidney.These results showed that immune-associated tissues,including mucosal lymphoid tissues,had the cells to conduct a special immune response.The number of immune cells was significanty different among immune-associated tissues of turbot. 大菱鲆 免疫相关组织 免疫球蛋白阳性细胞 分布 turbot（Scophthalmus maximus L.） immune-associated tissues immunoglobulin-positive cell distribution S941 国家自然科学基金资助项目（30771648）;国家高技术研究发展计划（863）项目（2006AA100306）;天津市自然科学基金资助项目（07JCYBJC11900）;天津市科技发展项目（06YFGZNC001300） 2008-06-01 2021-04-01 6 54