荧光光谱法研究抗癌药物白藜芦醇与人血清白蛋白相互作用

荧光光谱法研究抗癌药物白藜芦醇与人血清白蛋白相互作用 Interaction of Anticancer Drug Resveratrol and Human Serum Albumin by Fluorescence Spectroscopy 吕中 L Zhong1 1 2 first-author 黄杰 HUANG Jie2 1 2 户业丽 HU Yeli2 1 2 程波 CHENG Bo2 1 2 张佑红 ZHANG Youhong2 1 2 邹国林 ZOU Guolin1 1 2 武汉大学生命科学学院/病毒学国家重点实验室 武汉大学生命科学学院/病毒学国家重点实验室 College of Life Sciences,Wuhan University/State Key Laboratory of Virology College of Life Sciences,Wuhan University/State Key Laboratory of Virology 武汉工程大学化工与制药学院/绿色化工过程教育部重点实验室 武汉工程大学化工与制药学院/绿色化工过程教育部重点实验室 School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology/Key Laboratory forGreen Chemical Process of Ministry of Education School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology/Key Laboratory forGreen Chemical Process of Ministry of Education 运用荧光光谱法研究了抗癌药物白藜芦醇（resveratrol,Res）与人血清白蛋白（human serum albu-min,HSA）的相互作用.结果显示,在生理条件下,HSA使Res的荧光最大发射峰发生明显蓝移,说明药物与蛋白间发生相互作用.平衡透析结果表明,Res在HSA上只有一个结合位点.Res对HSA的荧光猝灭是静态猝灭过程,药物与蛋白之间形成复合物,结合常数为7.43×105L/mol（298 K）,结合距离为3.50 nm.8-苯胺基-1-萘磺酸（8-anilion-1-naphthalenesulfonic acid,ANS）结合研究及热力学分析结果表明Res结合在HSA的疏水腔内,疏水作用为主要结合力. The interaction of the anticancer drug resveratrol（Res） with human serum albumin（HSA） has been investigated by fluorescence spectroscopy.The fluorescence spectra show that the addition of HSA results in a distinct blue shift in the maximum emission peak of Res,which indicates that there is interaction between Res and HSA.Equilibrium dialysis results show that there is only one Res binding site on HSA.The fluorescence quenching of HSA induced by Res is static quenching procedure and a complex of Res and HSA may be formed.The binding constant is 7.43×105 L/mol-1at 298 K.The distance between Res and HSA is 3.50 nm.8-Anilion-1-naphthalenesulfonic acid binding studies and thermodynamic analysis suggest that Res binds to the hydrophobic cavities of HSA,and hydrophobic interaction plays a major role in the binding of Res to HSA. 白藜芦醇 人血清白蛋白 相互作用 荧光光谱 resveratrol human serum albumin interaction fluorescence spectroscopy R285;R287 国家自然科学基金（30670464）;武汉工程大学校长基金资助项目 2008-04-01 2021-04-01 4