凡纳滨对虾组织蛋白酶L基因的单核苷酸多态性分析

凡纳滨对虾组织蛋白酶L基因的单核苷酸多态性分析 SNP Analysis of the Cathepsin L Gene in Litopenaeus vannamei 马宁 MA Ning 1 first-author 陈晓汉 CHEN Xiaohan 1 曾地刚 ZENG Digang 1 彭敏 PENG Min 1 李咏梅 LI Yongmei 1 广西水产研究所 广西水产研究所 Guangxi Institute of Fisheries Guangxi Institute of Fisheries 采用PCR产物直接测序法,对凡纳滨对虾40份DNA样本的组织蛋白酶L基因进行单核苷酸多态性（SNP）检测.经过对测序结果统计分析,共发现SNP 20个,分别是:A150C,T226G,G240A,C429T,T453C,G537A,C597T,C645T,G798C,C831T,C955T,C963T,C977T,C982T,G1001C,A1005T,C1117T,C1132T,G1367A,T1391C.其中6个SNP是外显子中的错义突变,3个SNP是外显子中的同义突变,11个SNP是内含子中的突变.本检测为进一步进行凡纳滨对虾生长性状关联研究提供了有用信息. Forty shrimp（Litopenaeus vannamei） DNA samples were inspected for single nucleotide polymorphism（SNP） in Cathepsin L gene by direct sequencing of PCR production.Twenty SNP sites were detected,namely,A150C,T226G,G240A,C429T,T453C,G537A,C597T,C645T,G798C,C831T,C955T,C963T,C977T,C982T,G1001C,A1005T,C1117T,C1132T,G1367A and T1391C,in which 6 SNP sites were missense mutations in exon region,3 SNP sites were synonymous mutations in exon region,and 11 SNP sites were mutations in intron region.These findings provide important data for the growth trait association study of Litopenaeus vannamei. 凡纳滨对虾 组织蛋白酶L基因 单核苷酸多态性 Litopenaeus vannamei cathepsin-L gene single nucleodide polymorphisms（SNP） S917.4 国家科技支撑计划项目（2006BADOIA13）;广西“新世纪十百千人才工程”专项资金（2007113）;广西自然科学基金（桂科青0542026）资助项目 2008-04-01 2021-04-01 4