长期慢性强迫游泳应激抑郁模型大鼠前额叶脑代谢物的¹H NMR研究

长期慢性强迫游泳应激抑郁模型大鼠前额叶脑代谢物的 1 H NMR研究 Cerebral Metabolic Changes in Prefrontal Cortex of Depression-Like Rat Model with Long-Term Chronic Forced Swimming Studied by ex vivo High Resolution 1 H NMR 李春霞 LI Chunxia 1 1 first-author 王亚强 WANG Yaqiang 1 1 潘文举 PAN Wenju 1 1 向赟 XIANG Yun 1 1 雷皓 LEI Hao1 1 中国科学院武汉物理与数学研究所/波谱与原子分子物理国家重点实验室 中国科学院武汉物理与数学研究所/波谱与原子分子物理国家重点实验室 Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences/State Key Laboratory ofMagnetic Resonance and Atomic and Molecular Physics Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences/State Key Laboratory ofMagnetic Resonance and Atomic and Molecular Physics 通过离体高分辨磁共振氢谱（1H NMR）技术结合行为学测试研究,发现大鼠经慢性强迫游泳应激（CFSS）4周后出现了与临床重型抑郁症患者类似的代谢和行为异常表现:前额叶（PFC）谷氨酸系统脑代谢物如谷氨酸（Glu）、谷氨酰胺（Gln）及牛磺酸等出现显著减低;出现快感缺失、精神运动抑制及体重减轻等抑郁样行为.结果表明4周CFSS大鼠模型是一种可用于研究针对中枢谷氨酸系统异常的抗抑郁药及探讨抑郁致病机制的重要动物模型. To evaluate the face and construct the validity of the depression-like rat model with long-term（4 weeks） chronic forced swimming（CFSS）,the cerebral metabolic changes of prefrontal cortex（PFC） were studied by ex vivo high resolution 1H NMR as well as behavior tests.The results showed that,relative to control,the CFSS rats had shown significantly reduced glutamate（Glu）,glutamine（Gln）,taurine levels in their PFC.Behavioral data showed several scores of CFSS rats had significantly reduced involving in relative sucrose intake and sucrose preference in sucrose test,rearing score in the open field test and weight gain compared to the control rats.In a word,these results suggest that long-term CFSS treatment can induce obvious decrease in the metabolism of Glu,Gln and Glx（Glu+ Gln） in PFC and evident behavioral abnormalities resembling patients of major depressive disorder.The long-term CFSS model might be used to study antidepressants specifically targeting the central glutamatergic system and the underlying mechanisms of clinical depression. 高分辨磁共振氢谱 抑郁症 慢性强迫游泳应激 谷氨酸 前额叶 大鼠 high resolution 1H NMR depression chronic forced swimming stress glutamate prefrontal cortex rat R749.4 国家重点基础研究发展计划（973）项目（2006CB705607）;国家高技术研究发展计划（863）项目（2006AA02Z4A1） 2008-04-01 2021-04-01 4