间接荧光猝灭法测定β-内酰胺类抗生素

间接荧光猝灭法测定β-内酰胺类抗生素 Indirect Spectrofluorimetric Determination of β Lactam Antiotics 潘祖亭 PAN Zu ting 1 first-author 王润涛 WANG Run tao 1 徐勉懿 XU Mian yi 1 武汉大学化学学院 武汉大学化学学院 College of Chemistry, Wuhan University College of Chemistry, Wuhan University 系统地研究了在ＴｒｉｔｏｎＸ－１００存在下的弱碱性介质中，羧苄青霉素、氨苄青霉素、长效青霉素和头孢菌素ＶＩ对２，７－二溴羟汞基荧光黄钠盐的荧光定量猝灭作用，提出了一种间接荧光猝灭测定β－内酰胺类抗生素含量的新方法．该法简便、快速，体系稳定，线性范围宽，并已成功地用于体液中β－内酰胺类抗生素残留物的检测，结果令人满意 An indirect, simple and highly sensitive spectrofluorimetry has been investigated for determination of β lactam antiotics. In the presence of suitable amount of Triton X 100, the excitation and emission wavelength are at 470 nm and 548 nm respectively. The linear range of carbenicillin, ampicillin, benzathine and cephalosporin are 0～10 mg/L, 0～10 mg/L, 0～8 mg/L and 0～12 mg/L. The method has been applied to determine trace amount of β lactam antiotics with satisfactory results. 抗生素 荧光光谱法 表面活性剂 药物 β lactam antiotics spectrofluorimetry surface active agent pharmaceuticals O657.39 国家自然科学重点基金 1999-02-01 2021-04-01 2