牛早期胚胎性别鉴定的PCR方法研究

牛早期胚胎性别鉴定的PCR方法研究 Study on the Sex Identification of Bovine Early Embryos by PCR 吕碧文 LU Bi wen 1 1 2 3 first-author 俞颂东 2 1 2 3 金水仙 YU Song dong 1 1 2 3 浙江省农科院畜牧兽医所 浙江省农科院畜牧兽医所 1 Institute of Animal Science,Zhejiang Academy of Agricultual Sciences 1 Institute of Animal Science,Zhejiang Academy of Agricultual Sciences 浙江丽水师范专科学校 浙江丽水师范专科学校 2 Lisui Teacher’s College 2 Lisui Teacher’s College 浙江省农科院畜牧兽医所浙江丽水师范专科学校 浙江省农科院畜牧兽医所浙江丽水师范专科学校 探讨了ＰＣＲ法鉴定牛早期胚胎性别时所用的引物，反应缓冲液中Ｍｇ２＋浓度，循环反应的次数及可能造成污染的各种因素对性别鉴定结果的影响．并对１８个胚胎进行了性别鉴定，移植３８枚性别鉴定后的胚胎，成功产下２头与预测性别一致的牛犊，胚胎性别可鉴率为９４．１％，预测性别符合率为１００％． We discussed several factors which would influence the results of sex identification of bovine early embryos by PCR,for example,PCR primers,the concentration of Mg 2+ in PCR buffer,the cycle numbers and calf serum in PBS buffer et al,and identified the sexes of 18 bovine early embryos respectively which had beed cutted to two parts for transfer.Under our condition,the identified efficiency of bovine early embryos was 94.1%. Two calf have borned,which have the same sex with our forecast by PCR. 胚胎性别鉴定 PCR S823.3;Q954.4 浙江省科委基金 1999-02-01 2021-04-01 2