癌胚抗原启动子驱动tk基因在人肺腺癌细胞中的靶向表达

癌胚抗原启动子驱动tk基因在人肺腺癌细胞中的靶向表达 Targeting Expression of HSVtk Gene Driven by CEA Promoter in Lung Cancer Cells 肖庚富 XIAO Geng fu 1 first-author 齐义鹏 QI Yi peng 1 程璇 CHENG Xuan hong 1 李凌云 LI Ling yun 1 武汉大学病毒研究所 武汉大学病毒研究所 Institute of Virology, Wuhan University Institute of Virology, Wuhan University 用荧光素酶报导基因，发现ｃｅａ启动子在ＣＥＡ阳性的肺腺癌细胞Ａ５４９中的活性是在ＣＥＡ阴性的宫颈癌细胞Ｈｅｌａ中的１６倍．构建了重组表达质粒ｐＣＥＡｔｋ，ｃｅａ启动子控制效应基因单纯疱疹病毒胸苷激酶基因（ＨＳＶｔｋ）的表达．转染了质粒ｐＣＥＡｔｋ的Ａ５４９细胞对甘昔洛韦（ＧＣＶ）的敏感性是未转染细胞的８６５倍，而转染了ｐＣＥＡｔｋ的Ｈｅｌａ细胞则仍保持对ＧＣＶ的抗性．结果表明：ｃｅａ启动子可用于ＣＥＡ阳性的肺腺癌等细胞的靶向性基因治疗 By using luciferase reporter gene, we found that promoter of carcinoembryonic antigen （CEA）gene exhibit 16 times high activity in CEA producing lung cancer cells, A549 than in nonproducing cells, Hela. We also constructed a recombinant expression plasmid pCEATK, in which CEA promoter drives the effector gene, thymidine kinase gene of Herpes Simplex Virus （HSVTK）. A549 cells transfected with pCEATK became 865 times more sensitive to ganciclovir （GCV） than the control cells. However, Hela cells transfected with this plasmid remained resistant to GCV. These data indicate the potential for targeted gene therapy using the CEA promoter against CEA producing tumor cells, such as lung cancer cells. 癌胚抗原 单纯疱疹病毒 胸苷激酶基因 基因治疗 carcinoembryonic antigen herpes simplex virus thymidine kinase gene gene therapy Q789 国家自然科学基金,湖北省自然科学基金 1999-02-01 2021-04-01 2