By the means of mixed culture and CBS mixed culture media

Clostridium butyricum and Lactobacillus rhamnosus are mixed-cultured together under the condition of 37 ℃.The findings of the test showed that these two have good co-existing relationship in mixed culture system.The biomass in mixed culture reaches 6.3×108

43.5×108 CFU/mL

increasing by 80% and 51.7% respectively compared with that in pure culture

and mixed culture has stronger bacteriostatic activity for the common intestinal pathogenic bacterium

and the bacteriostatic activity sequence as Colibacillus

Sh.dysenteriae and Salmonella.The experiment shows that this method possesses perfereable feasibility and practicability.