To evaluate the toxicological safety of recombinant Sacchromyces cerevisiae expressing salmon calcitonin

tests included acute toxicity test

micronucleus test

sperm shape abnormality test

traditional teratogenicity test and 30 days feeding test were performed.Results showed oral LD50 was higher than 10.0 g/kg both in male and female mice.Micronucleus test and Sperm shape abnormality test were negative.There was no maternal toxicity or embryotoxicity in teratogeniccity test.No significant toxicity was detected in 30 days feeding test.Conclusion could be drawn that recombinant Saccharomyces cerevisiae expressing salmon calcitonin has no negative influences on the animal

which proves that it is safe and nontoxic.