A mesophilic hydrogen production strain

designated as 08-1

was isolated from 10 L continuous stirred tank reactor（CSTR） and identified as a strain of Clostridium sp.based on its phenotype and 16S rRNA gene.Effects of concentration and type of carbon sources

temperature and pH value control on hydrogen production by strain 08-1 have also been investigated.Sucrose

biomass and components complicated wastes were favorable carbon sources for growth and hydrogen production of strain 08-1.The optimum temperature and pH was 40 ℃ and 5.5

respectively

for hydrogen production of strain 08-1.Under batch fermentative hydrogen production conditions of sucrose concentration of 20 g/L

temperature at 40 ℃

pH at 5.5 controlled and stirred rate at 100 r/min

the maximal hydrogen yield and volumetric hydrogen production rate for strain 08-1 were 3.06 mol and 245 mL·（L·h）-1

respectively.The results demonstrate that the strain possesses potential application in biohydrogen production from organic wastes.