By disc-agar diffusion method and broth microdilution susceptibility testing

the antimicrobial activity in vitro of the oil was evaluated against 2 fungi and 10 bacteria including 5 clinical isolated strains.The results showed that the oil presented a broad antimicrobial spectrum

and had better antimicrobial activity against microzyme and Gram-positive bacteria

the minimal inhibitory concentration（MIC） values were 0.02

0

.31 g/L and the minimum bactericidal concentration（MBC） values was 0.04

1

.25 g/L.The essential oil of subterranean part of Artemisia imponens was then analyzed by GC-MS.The results show that twenty-eight identified components in the oil mainly monoterpene and sesquiterpene accounts for 81.41% of tatal

and Vulgarnoe B（26.58%）

Cineole（1

8-）（19.89%） and Camphor（7.91%） are the key components.