卵母细胞的卵龄对猪核移植效率的影响

卵母细胞的卵龄对猪核移植效率的影响 Effect of Recipient Porcine Oocytes on Nuclear Transfer 陈乃清 CHEN Nai qing 1 1 2 3 first-author 赵浩斌 ZHAO Hao bin 2 1 2 3 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 湖北省农业科学院畜牧兽医研究所,武汉,430209 湖北省农业科学院畜牧兽医研究所,武汉,430209 The Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences The Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences 武汉大学生命科学学院!武汉430072湖北省农业科学院畜牧兽医研究所,武汉,430209 武汉大学生命科学学院!武汉430072湖北省农业科学院畜牧兽医研究所,武汉,430209 对核移植中，猪卵母细胞的卵龄对核移植效率的影响进行了研究结果表明：体外成熟培养 ４８ ｈ 的猪卵母细胞最适合作为核受体，其激活率达 ７８．３％ ，并无裂解；去核率高达９０％ ；在作为核受体进行核移植时，融合率为７５％ ；重构胚胎的卵裂率为６４．６％ 卵龄进一步增大，激活率略有增加，但去核率、融合率和卵裂率均下降 Studies were carried out to find out the optimal age of recipient porcine oocyte for successful nuclear transfer. The results indicated that: when oocytes cultured in vitro for 48 hours, 1. Parthenogenetical activation rate was high to 78.3% after electrostimulus; 2. Enucleation rate of oocytes was high to 90%; 3. Fusion rate and development rate of the restructured embryos was respectively 75% and 64.6%. Along with age of oocyte increase, enucleation rate of oocytes and development rate of the restructured embryos would decrease; enucleation rate of oocytes and fusion rate were no significantly change. As a result, it is recommended that oocytes cultured in vitro for 48 hours were optimal oocyte for successful nuclear transfer. 核移植 卵龄 猪 卵母细胞 nuclear transfer egg age pig oocytes 国家自然科学基金(61672102) 1999-04-01 2021-04-01 4