AFLP在水稻线粒体DNA研究中的应用

AFLP在水稻线粒体DNA研究中的应用 Utilization of AFLP in the Study of Rice mtDNA 凌杏元 LING Xing yuan 1 first-author 关和新 GAN He xin 1 周培疆 ZHOU Pei jang 1 朱英国 ZHU Ying guo 1 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 对 Ａ Ｆ Ｌ Ｐ应用于水稻线粒体基因组研究中的问题进行了研究结果表明： Ａ Ｆ Ｌ Ｐ分析对ｍ ｔ Ｄ Ｎ Ａ 质量要求高，ｍ ｔ Ｄ Ｎ Ａ 应完整并反复抽提以达高纯度 ｍ ｔ Ｄ Ｎ Ａ 不完全酶切所造成的假阳性可经第二次酶切排除选择引物一个选择碱基最适合水稻ｍ ｔ Ｄ Ｎ Ａ Ａ Ｆ Ｌ Ｐ分析 另外，研究表明从银染后未经烘烤的新鲜银染凝胶回收的 Ｄ Ｎ Ａ 扩增效果好、产物经纯化可直接用于克隆以及探针标记 Some problems in the use of AFLP to the analysis of rice mtDNA have been studied. The results show that the mtDNA used for the study of AFLP should be integrity and be extracted repeatedly to high purity. The artificial positive bands results from partially digestion of mtDNA can be solved by the second restriction digestion. One selective nucleotide of selective primer is most suitable for AFLP assay of rice mtDNA. Besides, the DNA that isolated from the freshly stained gel can be amplified under high stringency PCR conditions, the amplified product can be used for cloning and probe labering directly. 水稻 线粒体DNA 扩增片段长度多态性（AFLP） rice mitochondria DNA （mtDNA） amplified fragments length polymorphism （AFLP） S511.03 国家自然科学基金(61672102) 1999-04-01 2021-04-01 4