电脉冲激活猪卵母细胞的研究

电脉冲激活猪卵母细胞的研究 Electro activation of the Porcine Oocytes 陈乃清 CHEN Nai qing 1 1 2 3 first-author 赵浩斌 ZHAO Hao bin 2 1 2 3 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 Collage of Life Sciences, Wuhan University. Collage of Life Sciences, Wuhan University. 湖北省农业科学院畜牧兽医研究所,武汉,430209 湖北省农业科学院畜牧兽医研究所,武汉,430209 Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Sciences. Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Sciences. 武汉大学生命科学学院!武汉430072湖北省农业科学院畜牧兽医研究所,武汉,430209 武汉大学生命科学学院!武汉430072湖北省农业科学院畜牧兽医研究所,武汉,430209 通过对猪卵母细胞的电激活进行系统研究，结果表明：对于体外成熟卵母细胞，电场强度为 １ ２５０ Ｖ／ｃｍ ，脉冲时程为４０、６０ μｓ时，效果较好，一次脉冲激活率可达８５．３％ 和８７．１％ ；而对于体内成熟卵母细胞，电场强度为１ ５００ Ｖ／ｃｍ ，脉冲时程为４０、６０ μｓ 时，效果较好，一次脉冲激活率达８５．７％ 和８８．２％ ；增加脉冲次数并不能显著提高激活率，反而使卵母细胞裂解率显著增加；激活液选用０．３ ｍ ｏｌ／ Ｌ甘露醇（含０．１ ｍ ｍ ｏｌ／ Ｌ Ｍｇ Ｓ Ｏ４ ）时，添加 ０．１ ｍ ｍ ｏｌ／ Ｌ Ｃａ Ｃｌ２ ，获得较好的激活率（８７．８％ ）电脉冲激活卵母细胞后进一步培养，体内和体外成熟卵母细胞的发育率并无显著差异 The techniques on electro activation of the porcine oocytes were systematically studied. In the study of electroactivation, parthenogenetical activation rate was high to 85.3%～87.1% when pulse were 40～60 μs, when direct current field strength was 1 250 V/cm for oocytes maturated in vitro; and 85.7% 8 8.2% when direct current field strength was 1 500 V/cm for oocytes matured in vivo. Multiple pulses could not increase significantly parthenogenetical activation rate, but increased oocytes lysis rate. When nonelectrolyto solution （0.3 mol/L Mannital +0.1 mmol/L MgSO 4+0.1 mmol/L CaCl 2） was used as electro activation medium, parthenogenetical activation rate was high to 87.8%. 电激活 卵母细胞 猪 electro activation oocytes pig 国家自然科学基金(61672102) 1999-04-01 2021-04-01 4