精子介导的报道基因在转基因泥鳅胚胎中的表达

精子介导的报道基因在转基因泥鳅胚胎中的表达 Expression of Sperm mediated Reporter Gene in Embryos of Transgenic Loach 常洪 CHANG Hong 1 first-author 余其兴 YU Qi xing 1 周荣家 ZHOU Rong jia 1 郭一清 GUO Yi qing 1 刘利 LIU Li 1 吴海 WU Hai 1 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 以含ｌａｃ Ｚ基因的质粒 ｐ Ｃ Ｈ１１０ 为外源 Ｄ Ｎ Ａ，采用外源 Ｄ Ｎ Ａ 和精子在保存液里混合保温处理，再进行精、卵的体外受精的方法，观测了精子介导的报道基因在转基因泥鳅胚胎中的表达，４ 次重复实验均获得了较稳定的实验结果 有 ９．６％ 的个体呈现ｌａｃ Ｚ基因表达的阳性对有关实验结果进行了分析和讨论 We used plasmid pCH110 which contained a lacZ reporter gene as foreign DNA, and mature female loach （Misgurnus anguillicaudatus Cantor） eggs were in vitro fertilized with loach sperm cells incubated with foreign DNA in a solution containing 2.9% glucose, 1% sodium citrate, 0.2% sodium bicarbonate, 0.03% potassium chloride and 30×10 3 μg/L bovine serum albumin. Then we detected the expression of sperm mediated reporter gene in embryos of transgenic loach. The experiment was made four times,and the result was relatively stable.9.6% of the treated embryos of loach showed positive which expressed lacZ gene. The result of experiment was analysed and discussed. 泥鳅精子 LacZ基因 表达 转基因 sperm of loach （Misgurnus anguillicaudatus Cantor） LacZ gene expression transgene Q789 国家“863”青年科学基金 1999-04-01 2021-04-01 4