博莱霉素切割DNA反应的定量测定及其影响因素

博莱霉素切割DNA反应的定量测定及其影响因素 Quantitative Determination and Effect Factor of DNA Degradation by Bleomycin 林元藻 LIN Yuan zao 1 1 2 first-author 邹国林 ZOU Guo lin 2 1 2 李鹏 LI Peng 2 1 2 广东药学院生物化学教研室,广州,510224 广东药学院生物化学教研室,广州,510224 Department of Biochemistry, Guangdong College of Pharmacy Department of Biochemistry, Guangdong College of Pharmacy 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 用硫代巴比妥酸反应分析了博莱霉素 Ａ５ （ Ｂ Ｌ Ｍ Ａ５ ）对 Ｄ Ｎ Ａ 的断裂作用在 Ｆｅ２＋ 和 Ｏ２存在下， Ｂ Ｌ Ｍ Ａ５能导致 Ｄ Ｎ Ａ 链断裂研究了一些因素对 Ｂ Ｌ Ｍ Ａ５ 催化 Ｄ Ｎ Ａ 断链反应的影响，设计并建立了 Ｂ Ｌ Ｍ Ａ５ 切割 Ｄ Ｎ Ａ 反应的定量测定方法，并测得 Ｋｍ 约为１５３ μｍ ｏｌ· Ｌ－ １， Ｖｍ ａｘ 约为００４６ Ａ５３２ｎｍ Ｕｎｉｔ·ｓ－ １  Cleavage action of DNA by bleomycin A 5（BLMA 5） was determined by thiobarbituric acid reaction. Breaks of DNA could be induced by BLMA 5 in the presence of Fe 2+ and O 2. Some effect factors of DNA degradation by BLMA 5 were studied. The quantitative determination method of DNA degradation by bleomycin were designed and established, and K m is 153 μmol·L -1 and V max is 0 046 A 532nm Unit·s -1 . 博莱霉素 切割DNA反应 定量测定 bleomycin DNA degradation quantitative determination Q586;R913 国家自然科学基金(61672102) 1999-04-01 2021-04-01 4