抑癌基因bcl-2在玉米染色体中的荧光原位杂交定位

抑癌基因bcl-2在玉米染色体中的荧光原位杂交定位 Physical Location of Tumor Suppressor Gene bcl 2 in Maize Chromosomes by FISH 杨征 YANG Zheng 1 first-author 蔡陈 CAI Chen leng 1 郭一清 GUO Yi qing 1 宋运淳 SONG Yun chun 1 刘立华 LIU Li hua 1 张后今 ZHANG Hou jin 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 以人的抑癌基因ｂｃｌ－２为探针，通过Ｓｏｕｔｈｅｒｎ杂交技术，发现ｂｃｌ－２在玉米和水稻的基因组中均具有同源序列；并运用荧光原位杂交技术，对玉米中的ｂｃｌ－２同源序列进行了定位．结果表明：ｂｃｌ－２在玉米第４号和第７号染色体上均检出了杂交信号，信号检出率分别为８．１６％和６．６３％．ｂｃｌ－２在第４号染色体长臂上的杂交信号与着丝粒的百分距离为５８．０７±４．９０，在第７号染色体长臂上的杂交信号与着丝粒的百分距离为８０．３４±３．１２．上述研究结果为研究植物细胞程序性死亡的可能调控机制提供了线索，将有利于进一步分析植物与动物细胞程序性死亡的相关性 To analyze the presence of homologous sequences of human tumor supprossor gene bcl 2 in maize and rice, Southern hybridization was performed and positive signals were detected in these two species’ genomes. FISH also was carried out for physical location of the bcl 2 homologous sequences in maize chromosomes. bcl 2 was hybridized onto the long arm of the fourth and the seventh chromosomes. The detection rates of FISH were 8.10% and 6.63%. The percentage distances from centromere to detection sites were 58.07±4.90 and 80.34±3.12 respectively. These results may provide important clues of the further research of the molecular mechanisms regulating the plant programmed cell death, and it may also benefit to further research on the relationship of programmed cell death between plants and animals. 玉米 细胞程序性死亡 bcl－2 同源序列 荧光原位杂交 maize programmed cell death bcl 2 homologous sequences FISH Q75 教育部博士点基金 1999-02-01 2021-04-01 2