黑色素对流感病毒诱导细胞凋亡的抑制效应

黑色素对流感病毒诱导细胞凋亡的抑制效应 Inhibition of Melanin From Pseudomonas maltophilia on the Apoptosis Induced by Influenza Virus in MDCK Cells 郑从义 ZHENG Cong yi 1 first-author 胡国斌 HU Guo bing 1 屈三甫 QU San pu 1 彭方 PENG Fang 1 段晓红 DUAN Xiao hong 1 彭珍荣 PENG Zhen rong 1 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 Cellege of Life Sciences, Wuhan University Cellege of Life Sciences, Wuhan University 用荧光染色技术及流式细胞仪分析方法研究了 Ａ１／京防８６１ 和 Ｂ／沪防９３１ 两株流感病毒感染与细胞凋亡的关系，探讨利用嗜麦芽假单胞菌黑色素抑制流感病毒诱导 Ｍ Ｄ Ｃ Ｋ 细胞凋亡的可能性 结果显示：黑色素在 １００ ｍ ｇ· Ｌ－ １ 浓度范围内，对 Ｍ Ｄ Ｃ Ｋ 细胞无细胞毒性； Ａ１／京防 ８６１ 和 Ｂ／沪防 ９３１ 两株流感病毒均可诱导 Ｍ Ｄ Ｃ Ｋ细胞凋亡，但二者存在毒力差异（ｐ＜ ０．０５）病毒感染１２ ｈ 后，荧光染色可观察到典型的凋亡核形态，流式细胞仪则可检测到病毒诱导 Ｍ Ｄ Ｃ Ｋ 细胞产生的凋亡峰，且细胞凋亡率分别为３７．０２％ 和 ２７２９％ ；２０ ｍ ｇ· Ｌ－ １ 的黑色素可有效抑制两株流感病毒诱导细胞凋亡，使细胞凋亡指数从２５％ ～３５％ 降至３％ 以下 结果表明，黑色素可以抑制 Ａ 和 Ｂ型流感病毒诱导细胞凋亡 The situation of apoptosis induced by influenza virus （A1/Jingfang 86 1 and B/Hufang 93 1） in cultured MDCK cells was studied and the selective inhibitory effect of melanin from P.maltophilia on the apoptosis induced by influenza virus was investigated. The results showed that the cytotoxicity of melanin was lower and limit to concentration to MDCK cell line was 100 mg·L -1 . The apoptosis induced activity of influenza virus A1/Jingfang 86 1 strain was more potent than that of B/Hufang 93 1 strain （ p < 0.05）. In the range of 20 1 00 mg·L -1 , melanin was found to significantly （ p < 0.001） inhibit apoptosis induced by 64 hemagglutinating unit influenza virus. Analysis of the cells by single laser flow cytometry did not show the sub G1 peak （apoptosis peak）. Apoptosis index dropped below 3% from 25% 3 5% by fluorescence microscope detection. From all of these findings, we concluded that melanin form P.meltophilia was able to inhibit apoptosis induced by influenza viru type A and B in MDCK cells. 嗜麦芽假单胞菌黑色素 流感病毒 细胞凋亡 melanin from P.meltophilia influenza virus apoptosis Q939.4 国家教委重点项目资金资助项目 1999-04-01 2021-04-01 4