菲咯啉铜配合物对体外DNA损伤的研究

菲咯啉铜配合物对体外DNA损伤的研究 DNA Damage in the System Containing Phenanthroline、Cu 2+ and Reductant in Vitro 邹国林 ZOU Guo\|lin 1 first-author 张帆 ZHANG Fan 1 欧荣 OU Rong 1 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Sciences,Wuhan University,Wuhan 430072,China) \ \ College of Life Sciences,Wuhan University,Wuhan 430072,China) \ \ 菲咯啉（phen）、Cu2+ 、还原剂体系在435 nm 出现特征吸收峰,表明体系中产生了（phen）2Cu+ ,维生素C（Vc）比巯基乙醇作还原剂时（phen）2Cu+ 产量更大.·OH 清除剂对phen、Cu2+ 、还原剂体系损伤DNA 无明显影响,但抑制对脱氧核糖的损伤.Vc为还原剂的体系中加入失活的超氧化物歧化酶（SOD）或小牛血清白蛋白对反应无影响,而活性SOD有显著抑制作用. 利用顺磁共振（ESR）技术证实,加入活性SOD或·OH清除剂可淬灭体系中·OH ESR信号. 以上结果表明,（phen）2Cu+ 是一种小分子催化剂,·OH是该体系造成DNA 损伤的重要因子. The production of （phen） 2Cu + in the system containing phenanthroline,Cu 2+ and reductant was detected by absorption spectrum around 435nm,and the yield of （phen） 2Cu + was higher in the vitamin C （Vc） system than that in the RSH system. ·OH scavenger showed little affect on the damage to DNA in the system, but explicitly inhibited the damage to the deoxyribose. The addition of inactive SOD or BSA to the system containing Vc as the reductant did not inhibit the reaction, but natural SOD did. ESR spectrum approved that natural SOD and ·OH scavenger could scavenge ·OH in the system .These results suggested that （phen） 2Cu + was a small molecular catalyst and ·OH was an important factor that made damage to DNA.\; 菲咯啉铜配合物 DNA损伤 羟自由基 电子顺磁共振 phenanthroline copper chelate DNA damage hydroxyl free radical electron spin resonance Q504 国家自然科学基金!（39770200） 1999-06-01 2021-04-01 6