伪狂犬病毒糖蛋白H基因片段的克隆、序列测定和分析

伪狂犬病毒糖蛋白H基因片段的克隆、序列测定和分析 Cloning Sequencing and Structrual Analysis of the Glycoprotein H Gene of A Wild Chinese Pseudorabies Virus Strain 潘兹书 PAN Zi\|shu 1 first-author 张楚瑜 ZHANG Chu\|yu 1 赵伟光 ZHAO Wei\|guang 1 丁建华 DING Jian\|hua\ 1 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Sciences, Wuhan University, Wuhan 430072,China)\ College of Life Sciences, Wuhan University, Wuhan 430072,China)\ 对伪狂犬病毒湖北地方株 （PRV HB株 ）糖蛋白 H基因片段进行了扩增、克隆、序列测定和分析 ,比较了该序列与 PRV Ka株及 NIA- 3株三者之间的同源性 .结果显示 ,克隆片段长 1396 bp,G+C含量 75 .6 % ,包括糖蛋白 H （g H ） N端 2 83个氨基酸编码区及上游调控序列和胸苷激酶 （TK） C端 136个氨基酸编码区 .g H基因ORF上游调控区存在有 TATA框和 CAT框的真核启动子特征结构 .PRV HB株 g H基因片段序列与 PRV Ka株和 NIA- 3株相应序列的核苷酸同源性相同 ,为 99.6 % .推导的 g H多肽 N端第 1～ 30位氨基酸组成的肽段富含疏水性氨基酸 ,具有真核信号肽的序列特征 . With pured PRV genome DNA as template, the part region of glycoprotein H gene and the upstream tk gene located with genomic BamHI fragments 11 and 16 was amplified by PCR, and its nucleotide sequence was determined by the dideoxy\|mediated chain termination method. A analysis of the nucleotide sequence and its deduced amino acid seqnence was performed with computer programs. The results revealed that the region of DNA sequenced was 1396bp and had the content of （G+C） 75.6%. The nucleotide sequence comparison of gH gene showed that the homology of the nucleotide sequence of PRV HB strain with PRV Ka strain and NIA\|3 strain was both 99.6%. The deduced amino acid sequence of gH polypeptide exhibited a strongly hydrophobic region present at the N\|terminus at position 1 3 0 amino acids, and exhibited features typical for eucaroytic signal sequences. 伪狂犬病毒 糖蛋白H基因 基因克隆 pseudorabies virus glycoprotein H gene gene clone 10.14188/j.1671-8836.2000.02.020 Q78 武汉市科技攻关重点项目!（ 962 0 0 10 18） 2000-02-01 2021-04-01 2