低温处理增强紫外照射诱导HeLa细胞凋亡

低温处理增强紫外照射诱导HeLa细胞凋亡 Hypothermia-Enhanced the Apoptosis Induced by Ultraviolet Irradiation in HeLa Cells 张小榕 ZHANG Xiao-rong 1 first-author 郑从义 ZHENG Cong\|yi 1 屈三甫 QU San\|fu 1 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Science, Wuhan University College of Life Science, Wuhan University 报道了紫外照射诱导体外培养的 He L a细胞凋亡的研究结果 ,对亚致死低温处理增强紫外照射诱导细胞凋亡的可能性进行了探讨 .结果显示 :He L a细胞经紫外线照射 10 min后培养 6 h,细胞凋亡指数明显升高 ,并于照射后 30～ 36 h达到高峰 （44 .82 %～ 70 .97% ） .荧光显微镜下观察可见细胞核固缩、碎裂 ,DNA凝胶电泳出现特征型的梯状图谱 ;低温处理后立即照射 ,可明显增加细胞凋亡指数 .但此效应是暂时性的 ,将 4℃处理了 12 h的细胞恢复于 37℃培养 2 4h再行照射 ,细胞凋亡指数与纯照射组相比并无明显改变 （P>0 .0 5 ） . Ultraviolet（UV） irradiation induced apoptosis in HeLa cells was reported. In addition, sublethal hypothermia\|enhanced the apoptosis induced by UV was studied. The results revealed: The apoptosis index （AI） began to rise after irradiated HeLa cells had been in culture for 6 h and reached summit （44.82% 7 0.97%） during 30 3 6 h after irradiation. HeLa cells went through nuclear condensation and fragmentation. Agerose gel electrophoresis of DNA extracted from irradiated cells revealed typical ladder pattern. Hypothermia enhanced the apoptosis of HeLa cells induced by UV. However, the radiosensitizing effect was transient:if the cooled cells were returned to 37℃ for 24 h before irradiation, there were no evident changes of AI compared with cells which underwent UV irradiation only （ P > 0.05）. 低温 紫外照射 细胞凋亡 hypothermia ultraviolet irradiation apoptosis 10.14188/j.1671-8836.2000.02.027 R730.5 国家教委重点项目资金资助课题!（ 94-170 ） 2000-02-01 2021-04-01 2