鱼腥藻sp.PCC 7120液泡的诱导和分离

鱼腥藻sp.PCC 7120液泡的诱导和分离 Induction and Isolation of Vacuoles from the Blue-green Alga Anabaena PCC 7120 郭厚良 GUO Hou\|liang 1 1 2 first-author 易平 YI Ping 1 1 2 浦秋文 PU Qiu\|wen 1 1 2 金传荫 JIN Chan\|yin 2 1 2 周云珍 ZHOU Yun\|zhen 1 1 2 杨清平 SON Wen\|zhen 1 1 2 宋文贞 1 2 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 1 College of Life Sciences, Wuhan University 1 College of Life Sciences, Wuhan University 中国科学院水生生物研究所,武汉,430072 中国科学院水生生物研究所,武汉,430072 2 Institute of Hydrobiology, the Chinese Academy of Sciences 2 Institute of Hydrobiology, the Chinese Academy of Sciences 将蓝藻培养于含 0 .0 5 mol/ L Na Cl的液体培养基 ,3d后细胞结构改变 ,出现无色透明区 .将此材料经溶菌酶处理形成原生质球 ,然后降低渗透压 ,原生质球破裂 ,液泡释放 .此液泡为极为标准的园球状 ,完全透明 ,泡体内无可辩物质 .电镜检查表明为一个单一膜所包围 ,泡内没有内囊体等细胞内物质 ,该膜亦显示典型三明治状单位膜结构 . The trichome were grown in the medium containing 0.05 mol/L NaCl for 3 d and over 90%, the trichome cells were vacuolated. Then the cells were treated with 1%（W/V） lysozyme containing complex salts [NaCl,KNO 3,（NH 4） 2SO 4 in phasphate buffer pH7] and the cells were transformed into vacuolated spheroplasts. Under a hypotonic condition the spheroplasts were broken and the vacuoles were released. The vacuoles were spherical, fully transparent and nonpigmentous. There were no any thylakoids and other cytomplasmic materials in it. An electromicroscopic examination showed that the vacuoles were formed by a single membrane and the membrane had a sandwich structure which belong to unit membrane. 鱼腥藻sp.PCC7120 无机盐 液泡 Anabaena PCC 7120 lnorganic salts vacuoles 10.14188/j.1671-8836.2000.02.030 Q949.2 国家自然科学基金资助项目!（ 3 9870 0 83 ） 2000-02-01 2021-04-01 2