硫唑嘌呤氧化反应的荧光光谱法研究

硫唑嘌呤氧化反应的荧光光谱法研究 Study on the Redox Reaction of Azathioprine by Spectrofluorimetry 黄春保 HUANG Chun\|bao 1 first-author 张丽瑶 ZHANG Li\|yao 1 曹启花 CAO Qi\|hua 1 潘祖亭 PAN Zu\|ting 1 武汉大学化学与环境科学学院,武汉,430072 武汉大学化学与环境科学学院,武汉,430072 College of Chemistry and Environment Science, Wuhan University College of Chemistry and Environment Science, Wuhan University 研究了硫唑嘌呤的水解产物与 KMn O4的氧化作用 ,提出了高灵敏测定硫唑嘌呤的荧光光度分析新方法 .在碱性介质中 ,将硫唑嘌呤通过沸水浴加热水解 - KMn O4氧化 ,使其转化成 6 -嘌呤磺酸钠 ,该产物不仅具有强的荧光且稳定性好 .本体系的激发波长为 2 86 nm,发射波长为 397nm,荧光强度 F与硫唑嘌呤的含量在 0 .0 1～5 .0 m g/ L 范围内呈线性关系 ,检测限为 0 .0 0 5 mg/ L.应用该法于片剂中硫唑嘌呤含量的测定 ,与标准方法对照 ,结果令人满意 . A new redox reaction\|spectrofluorimetry for the determination of azathioprine （6\|AP） in tablets was developed. The reaction proceeded completely in the presence of 2.0×10 -2 mol·L -1 potassium permanganate. The fluorescence was measured at 397 nm with excitation at 286 nm. The calibration curve was linear over the concentration range 0.01 5 .0 mg/L for 6\|AP in tablets and the results obtained were in agreement with those given by an official method. 荧光光谱法 硫唑嘌呤 药物分析 spectrofluorimetry azathioprine pharmaceutical analysis 10.14188/j.1671-8836.2000.04.003 O657.39 2000-04-01 2021-04-01 4