马氏珠母贝遗传多样性的RAPD分析

马氏珠母贝遗传多样性的RAPD分析 RAPD Analysis on Genetic Diversity of Pinctada martensii Dunker 王爱民 WANG Ai-min 1 1 2 first-author 邓凤姣 DENG Feng-jiao 2 1 2 张锡元 ZHANG Xi\|yuan 2 1 2 阎冰 YAN Bing 1 1 2 叶力 YE Li 1 1 2 毛勇 MAO Yong 1 1 2 张木先 ZHANG Mu-xian 2 1 2 广西海洋研究所,北海,536000 广西海洋研究所,北海,536000 Guangxi Ocean Institute Guangxi Ocean Institute 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Siences,Wuhan University College of Life Siences,Wuhan University 采用 OPM组 2 0个碱基随机引物对海南三亚 （SY） ,海南洋浦港 （YP） ,广西涠洲岛 （WZ）的 3个天然群体和广东雷州人工养殖群体 （L Z）的马氏珠母贝进行了 RAPD分析 .其群体内的平均遗传相似度 （similarity,S）分别是 0 .5 86 6 ,0 .6 5 14,0 .6 6 6 6 ,0 .715 8,由低到高依次为 SY<YP<WZ<L Z;群体内的遗传变异度 （variability,V）分别是 0 .4134 ,0 .34 86 ,0 .3334 ,0 .2 842 ,大小由高到低依次为 SY>YP>WZ>L Z.结果表示 :天然群体的遗传多样性大于养殖群体的遗传多样性 . The RAPD analysis was used to study the genetic diversity of three natural populations of pinctada martensii Dunker obtained from San Ya,Hai Nan Province（SY）,Yang Pu Harbor,Hai Nan Province（YP）,Wei Zhou island, Guang Xi Province（WZ） and a cultivated population obtained from Lei Zhou,Guang Dong Province（LZ）.The random primer containing 20 nucleotide of M group made in Operom Company was used in this study.The genetic Similarity of each population were 0.586 6,0.651 4,0.666 6, 0.715 8 respectively.and the order from small to big was SY, YP,WZ, LZ. The genetic variability of each population were 0.413 4,0.348 6,0.333 4, 0.284 2 respectively and the order from big to small was SY, YP,WZ, LZ. We can find that the genetic diversity of natural population was more than cultivated population. 马氏珠母贝 RAPD 遗传多样性 Pinctada martensii Dunker RAPD genetic diversity 10.14188/j.1671-8836.2000.04.019 Q346.5 国家自然科学基金!（ 3 9760 0 62 ）;广西自然科学区配基金!（ 982 40 0 9） 2000-04-01 2021-04-01 4