微生物酶催化甲胺磷降解机理初探

微生物酶催化甲胺磷降解机理初探 Primary Studies on Mechanism of Degrading Methamidophos by Microbe Enzyme 阮少江 RUAN Shao-jiang 1 1 2 first-author 刘洁 LIU Jie 1 1 2 王银善 WANG Yin\|shan 2 1 2 赵永芳 ZHAO Yong\|fang 1 1 2 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 1 College of Life Sciences, Wuhan University 1 College of Life Sciences, Wuhan University 中国科学院武汉病毒研究所,武汉,430071 中国科学院武汉病毒研究所,武汉,430071 2 Wuhan Institute of Virology, Chinese Academy of Sciences 2 Wuhan Institute of Virology, Chinese Academy of Sciences 甲胺磷 （MAP）农药经微生物酶 -甲胺脱氢酶催化后 ,采用聚酰胺薄膜层析、钼酸铵试剂和 HPL C等分法 ,分别可检测出氨基、磷酸根和甲醇等化合物 .将去除氨基的 MAP溶液作小鼠灌胃试验 ,发现该农药的毒性明显降低 .文中还对 MAP在自然界的降解过程等问题进行了讨论 . Amino group, methanol, phospho and sulfhydryl could be detected by proper methods after methamidophos pesticide was catalyzed by methylamine dehydrogenase （MADH）,one microbe enzyme. When methamidophos solution from which a mino group was removed poured stomach of mice, the toxicity of the pesticide reduced obviously. Related problems such as the degradation process of methamidophos in nature are also discussed. 微生物酶 甲胺磷降解 甲基营养菌 microbe enzyme methamidophos degradation methylotrophic bacteria 10.14188/j.1671-8836.2000.04.020 X172 国家自然科学基金!（ 3 9670 0 10 ） 2000-04-01 2021-04-01 4