p16蛋白对B型流感病毒诱导HeLa细胞凋亡的影响

p16蛋白对B型流感病毒诱导HeLa细胞凋亡的影响 Effect of p16 Protein Expression on HeLa Cell Apoptosis Induced by Influenza Virus B 郑从义 ZHENG Cong\|yi 1 first-author 吴海祥 WU Hai\|xiang 1 胡国斌 HU Guo\|bin 1 屈三甫 QU Shan\|pu 1 应松盛 YING Song\|sheng 1 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 以流感病毒 B/沪防 93- 1株感染 He L a细胞 ,通过 Hoechst332 5 8荧光染色、琼脂糖凝胶电泳分析检测细胞凋亡 ,并用免疫组化技术测定 p16蛋白的表达 ,探讨 p16蛋白表达对 He L a细胞凋亡的影响 .结果表明 ,B型流感病毒感染 He L a细胞可诱导其凋亡 ,感染 2 4h后 ,细胞凋亡数达 84.5 % ;He L a细胞凋亡伴随 p16蛋白的表达 ,2 4h达高峰 ,阳性率为 49.2 3± 1.70 .研究结果提示 ,B型流感病毒感染诱导 He L a细胞凋亡过程与 p16基因激活相关 ,p16蛋白可能是介导流感病毒诱导细胞凋亡的另一重要途径 . The methods of fluorescent staining and agrose gel electrophoresis were used to detect the induction of apoptosis by influenza virus strain B/Hufang 93\|1 in HeLa cells in vitro and the expression of apoptosis associated proteins were assayed by immunohistochemistry with monoclonal antibodies of corresponding antigens. The results showed that influenza/B virus is able to induced apoptosis in cultured HeLa cells,apoptotic cells was 84.5% infected for 24 h and expressed p16 protein at high levels simultaneously （Positive: 49.23±1.70）. These founds suggests that p16 protein may present a new pathway mediating apoptosis induced by influenza virus in tumor cells. B型流感病毒 HeLa细胞 细胞凋亡 p16蛋白 influenza virus B HeLa cell apoptosis p16 protein 10.14188/j.1671-8836.2000.04.022 Q939.4 国家教委重点项目经费!（ 94-170 ） 2000-04-01 2021-04-01 4